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April 15 寂寞拥挤在歡笑中掉淚 在呼吸中暈眩
在熱鬧中孤單 在人群中覺得累 凍得顫抖的夏天 寂寞擁擠的氣味 而我在回憶 捕捉失落的你 遺漏的章節 成為獨撐場面 青春劃成碎片 迴旋漫漫長街 愛的喘息如雷 我走遠 或許 我該道別 或許 如此最美 March 08 Sound of SilenceGreat songs will never fade. while I was watching Watchmen yesterday, this 40 year old song made my flesh creep, like a spark of light...
Hello darkness, my old friend
I've come to talk with you again Because a vision softly creeping Left its seeds while I was sleeping And the vision that was planted in my brain Still remains Within the sound of silence In restless dreams I walked alone Narrow streets of cobblestone 'Neath the halo of a street lamp I turned my collar to the cold and damp When my eyes were stabbed by the flash of a neon light That split the night And touched the sound of silence February 24 Beautiful Things - AndainNo one's calling for me at the door. And unpredictable won't bother anymore. And silently gets harder to ignore. Look straight ahead, there's nothing left to see. What's done is done, this life has got it's hold on me. Just let it go, what now can never be. I forgot that I might see, So many beautful things I forgot that I might need, to find out what life could bring. February 22 Stretch out your muscle Dr. Hermann Gaub talked about his current work on titin kinase stretching and single DNA assembly assay at this Thursday's symposium here at UIUC. They developed an experimental scheme where the AFM and TIRF microscopy were combined such that a nano-scaled pattern could be generated by arranging single fluorescently labeled DNA line after line using a AFM tip [1]. It looked kind of cool, but on second thougnt, i feel it seems no any application or question in reality would be addressed by this apparantly teeny fancy 'nanotechnique'. Contrarily, I am very into the titin kinase work, especially when he showed a movie zooming the human muscle into the molecular level step by step.
Biological responses to mechanical stress require strain-sensing molecules, whose mechanically induced conformational changes are relayed to signaling cascades mediating changes in cell and tissue properties. In vertebrate muscle, the giant elastic protein titin is involved in strain sensing via its C-terminal kinase domain (TK) at the sarcomeric M-band and contributes to the adaptation of muscle in response to changes in mechanical strain. TK is regulated in a unique dual autoinhibition mechanism by a C-terminal regulatory tail, blocking the ATP binding site, and tyrosine autoinhibition of the catalytic base. For access to the ATP binding site and phosphorylation of the autoinhibitory tyrosine, the C-terminal autoinhibitory tail needs to be removed. Here, we use AFM based single-molecule force spectroscopy, molecular dynamics simulations, and enzymatics to study the conformational changes during strain-induced activation of human TK. We show that mechanical strain activates ATP binding before unfolding of the structural titin domains, and that TK can thus act as a biological force sensor. Furthermore, we identify the steps in which the autoinhibition of TK is mechanically relieved at low forces, leading to binding of the cosubstrate ATP and priming the enzyme for subsequent autophosphorylation and substrate turnover. [2] February 15 后青春期的诗当烟雾随晨光飘散 枕畔的湖已风乾
期待已退化成等待 而我告别了突然 当泪痕勾勒成遗憾 回忆夸饰着伤感 逝水比喻 时光荏苒 终於 我们不再 为了生命狂欢 为爱情狂乱 然而青春彼岸 盛夏正要 一天 一天 一天的...灿烂 谁说不能让我 此生唯一自传 如同诗一般
无论多远未来 读来依然 一字 一句 一篇 都灿烂   February 14 Darwin's 200  Although I am not a fan for the suspicious evolutionary theory (well, the specific reasons are unpon request. lol.  ), here comes "the 200th anniversary of the birth of Charles Robert Darwin falls on 12 February 2009". "No single researcher has since matched his collective impact on the natural and social sciences; on politics, religions, and philosophy; on art and cultural relations. In this landmark year, our Nature news special provides continuously updated news, research and analysis on Darwin's life, his science and his legacy.''February 13 Birth and growth of a granular jetHeinrich Jaeger's talk was pretty interesting when he showed the liquid-like jet of solid particles. Thinking a flow of water falling down freely, the atoms are clustered as the flow drops, refering to 'water drop', followed by a jet of shoots upwards. However, strikingly when a bunch of heavy small particles are dropped onto a bed of loose, fine sand, a similar phenomenon occurs [1]. Surprisingly in contrast to the liquid situation, the granular jet exists in the absence of both surface tension and cohesion. How could that be? Why the surface tension is unnecessary to play the role? Heinrich used both simulation and camera recording to investigate the system in a quantitive way. It was funny when he described how his student had dropped the camera in parallel with the granular to the top of buffer foam.."Its xx thousand dollars, so we don't want to miss that",he said so. [1] John R. Royer et al., “Formation of Granular Jets Observed by High-Speed X-ray Radiography”, Nature Physics 1, 164-167 (2005). Interferometric 3D imaging with super resolution
I know its an insanely crazy idea to have three CCD cameras involved (probably 40k bulks for each). In current issue, a PNAS paper illustrated how a high spatial resolution method named iPALM could be achieved in a three-dimenional manner in terms of improving the previous PLAM/STORM imaging.A three-layered prism was introduced to form a multiphase interferometric scheme so that the Z-position could be resolved in a much sharper way by calibrating the inteference pattern among the signals of the three CCDs. The below is the superresolution iPALM image of COS7 cell expressing the membrane protein VSVG fused to td-EosFP, rendered with z axis color-coding. The insert impressively shows two distinct and well-separated Guassian peaks with ~10nm width in Z, which obviously pushed the visible light diffraction limit by one order of magnitude. Cell Biology: the force is with us  Believe it or not, cells and tissues can feel the mechanical force from the outside. Mechanosensitive adhesions mediated by membrane proteins called integrins are believed to underlie a number of these behaviors. Forces applied either by the cells’own cytoskeleton or from external sources induce strengthening of these adhesions and trigger a variety of intracellular signals through these effects. Two papers in current issues in Science, one by Friedland et al. and the other by del Rio et al.,increase our understanding of the molecular basis for this. This two pieces of amazing work attracted me because they're addressing a fundamental question how far nonequalibrium is prevailing in such a microscopic world and how the external forces get transmitted into the cell body via the cytoskeleton so that the inside molecules can actually feel the stretch. February 10 国境之南如果海会说话 如果风爱上砂
如果有些想念 遗忘在某个长假
我会聆听浪花 让风吹过头发
任记忆里的爱情在时间潮汐里喧哗
非得等春天远了 夏天才近了
我是在回首时终于懂得
当阳光 再次回到那 飘着雨的国境之南
我会试着把 那一年的故事 再接下去说完
当阳光 再次离开那 太晴朗的国境之南
妳会不会把 妳曾带走的爱 在告别前用微笑全归还
December 04 倔强－五月天当 我和世界不一样 那就让我不一样
坚持对我来说就是以刚克刚 我 如果对自己妥协 如果对自己说谎 即使别人原谅 我也不能原谅 最美的愿望 一定最疯狂 我就是我自己的神 在我活的地方 我和我最后的倔强 握紧双手绝对不放 下一站 是不是天堂 就算失望 不能绝望 我和我骄傲的倔强 我在风中大声的唱 这一次 为自己疯狂 就这一次我和我的倔强 对 爱我的人别紧张 我的固执很善良 我的手越肮脏 眼神越是发光 你 不在乎我的过往 看到了我的翅膀 你说被火烧过 才能出现凤凰 逆风的方向 更适合飞翔 我不怕千万人阻挡 只怕自己投降 我和我最后的倔强 握紧双手绝对不放 下一站 是不是天堂 就算失望 不能绝望 我和我骄傲的倔强 我在风中大声的唱 这一次 为自己疯狂 就这一次 我和我的倔强 October 08 Alanis Morissette - Not As WeReborn and shivering
Spat out on new terrain Unsure unconvincing This faint and shaky hour Day one day one start over again Step one step one I'm barely making sense for now
I'm faking it 'til I'm pseudo making it From scratch begin again but this time I as I And not as we Gun shy and quivering Timid without a hand Feign brave with steel intent little and hardly here Day one day one start over again Step one step one with not much making sense just yet I'm faking it til I'm pseudo making it From scratch begin again but this time I as I And not as we Eyes wet toward Wide open frayed If God's taking bets I pray He wants to lose Day one day one start over again Step one step one I'm barely making sense just yet I'm faking it til I'm pseudo making it From scratch begin again but this time I as I And not as we September 12 Declan Galbraith - Ego YouIn pain you speak all that you need When you’d decide When all is said and done You’d laugh it off and run In time you’ll see what you kept from me I should have known We could have made it through But that’s just ego you Time will show and time will tell What you need to make it well Time will show I know If I let you go Through all you life All filled with strife All you desire has been torn up in two Because of ego you |
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